Journal: Molecular cell
Article Title: An Mtb -Human Protein-Protein Interaction Map Identifies a Switch Between Host Anti-Viral and Anti-Bacterial Responses
doi: 10.1016/j.molcel.2018.07.010
Figure Lengend Snippet: (A) BMMs infected with Sendai virus (SeV) for 24h, and the relative number of virions in the supernatant were quantified by RT-qPCR; Mean ± SEM displayed. Phase-contrast image (10x) at 24h post-infection. Scale bars = 100 microns.
Article Snippet: RRID:AB_2258865 Mouse monoclonal anti-FLAG tag Sigma Cat #F1804 Mouse monoclonal anti-Strep tag IBA Life Sciences Cat # 2–1509-001 Bacterial and Virus Strains Mtb : CDC1551 Strain BEI Resources Cat # CNR-13649 Mtb : CDC1551, lpqN ::Tn himar1 BEI Resources Cat # NR-18454 Mtb : CDC1551, lpqN ::Tn himar1, attB MS6 ::pBUGFP, attB Tn5 ::pmv306Lux This paper N/A Mtb : CDC1551, lpqN ::Tn himar1 , attB MS6 ::pBU lpqN , attB Tn5 ::pmv306Lux This paper N/A Mtb : CDC1551, Rv0999 ::Tn himar1 TARGET Consortium Cat# JHU0999–616 Mtb : CDC1551, Rv0999 ::Tn himar1, attB MS6 ::pBUGFP, attB Tn5 ::pmv306Lux This paper N/A Mtb : CDC1551, Rv0999 ::Tn himar1 , attB MS6 ::pBU Rv0999 , attB Tn5 ::pmv306Lux This paper N/A Mtb : CDC1551, Rv1804c ::Tn himar1 BEI Resources Cat# NR-14706 Mtb : CDC1551, Rv1804c ::Tn himar1, attB MS6 ::pBUGFP, attB Tn5 ::pmv306Lux This paper N/A Mtb : CDC1551, Rv1804c ::Tn himar1 , attB MS6 ::pBU Rv1084c , attB Tn5 ::pmv306Lux This paper N/A Mtb : CDC1551, Rv2469c ::Tn himar1 BEI Resources Cat # NR-18289 Mtb : CDC1551, Rv2469c ::Tn himar1, attB MS6 ::pBUGFP, attB Tn5 ::pmv306Lux This paper N/A Mtb : CDC1551, Rv2469c ::Tn himar1 , attB MS6 ::pBU Rv2469 , attB Tn5 ::pmv306Lux This paper N/A Mtb : Erdman Strain W.R. Jacobs, Jr. BEI Cat # NR-15404 Mtb : Erdman, ΔeccCa 1 -ΔeccCb 1 , attB Tn5 ::pmv306Lux ( Rosenberg et al., 2015 ) N/A Mtb : Erdman::pmv261Zeo_MCherry This paper N/A Sendai virus Cantell Strain ATCC Cat# VR-907 Human herpesvirus 1 KOS, Strain 17 ATCC Cat# VR-1493 Chemicals, Peptides, and Recombinant Proteins Trypsin Promega V5280 Strep-Tactin Resin IBA Biosciences 2–1201-010 FLAG M2 Resin Sigma M8823–1ML Deposited Data All raw microscopy images and blots have been deposited to Mendeley Data ( http://dx.doi.org/10.17632/x3v7rb83bg.1 ) Experimental Models: Cell Lines Mouse: RAW267.4 ATCC TIB-71 Human: U937 ATCC CRL-1593.2 Human: Lenti-X 293T Clontech Cat# 632180 Human: 293T ATCC Cat # CRL-3216 Experimental Models: Organisms/Strains Mouse: C57BL/6 Jackson Laboratory Cat#: 000664 Mouse: B6 .Cg-Cblb tm1Hua , B6.
Techniques: Infection, Virus, Quantitative RT-PCR