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    ATCC strain cantell atcc
    Strain Cantell Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strain+cantell+atcc/Sendai+virus/pm41405988-572-157-159
    Average 95 stars, based on 179 article reviews
    strain cantell atcc - by Bioz Stars, 2026-09
    95/100 stars

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    Virus:

    Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH Santa Cruz Biotechnology Cat#: sc-32233; RRID: AB_627679 Anti-caspase-8 Cell Signaling Technology Cat#: 9746; RRID:AB_2275120 Anti-Cleaved PARP (Asp214) Cell Signaling Technology Cat#: 9541; RRID:AB_331426 Anti-3B (Anti-VPg EMCV ) This paper (Created by Immuno-Biological Laboratories Co., Ltd.) N/A (see Method Details) Anti-Mengo 3DPol Santa Cruz Biotechnology Cat#: sc-65633; RRID:AB_1565377 Anti-iNOS Santa Cruz Biotechnology Cat#: sc-7271; RRID:AB_627810 Anti-STAT1 Cell Signaling Technology Cat#: 9172; RRID:AB_2198300 Anti-pSTAT1 (Y701) Cell Signaling Technology Cat#: 9167; RRID:AB_561284 Anti-pSTAT2 (Y690) Cell Signaling Technology Cat#: 4441; RRID: AB_2198445 Anti-Myc-Tag Cell Signaling Technology Cat#: 2276; RRID: AB_331783 Anti-IκBα Santa Cruz Biotechnology Cat#: sc-371; RRID: AB_2235952 Anti-HSP70 Santa Cruz Biotechnology Cat#: sc-32239; RRID: AB_627759 Anti-IFNGR1 Proteintech Cat#: 10808-1-AP; RRID: AB_2121604 Anti-IFNGR2 Proteintech Cat#: 10266-1-AP; RRID: AB_2121709 Anti-mouse IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7056; RRID:AB_330921 Anti-rabbit IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7054; RRID: AB_2099235 Bacterial and virus strains Encephalomyocarditis virus (EMCV) ATCC Cat#: VR-129B Sendai Virus (SeV), strain Cantell ATCC Cat#: VR-907 Newcastle Disease Virus (NDV), strain Miyadera/51 Fujita et al., 1979 33 N/A Vesicular Stomatitis Virus (VSV), strain Indiana Oh et al., 2016 34 N/A Poliovirus (PV), strain type I Mahoney Shiroki et al., 1995 35 N/A Coxsackie Virus B3 (CVB3), strain Nancy Klump et al., 1990 36 N/A Enterovirus 71 (EV71), strain Isehara VP1145Q variant Kobayashi et al., 2018 37 N/A Competent high DH5α TOYOBO Cat#: DNA-903F Biological samples Human Dermal Fibroblasts, adult (HDFa) PromoCell Cat#: C-12302 Embryonated Chicken Eggs (9-day old) SHIMIZU Laboratory Supplies Co., Ltd. N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Nacalai Tesque Inc. Cat#: 16971-55 Fetal bovine serum (FBS) Sigma-Aldrich Cat#: 173012 Antibiotic-Antimycotic Mixed Stock Solution Nacalai Tesque Inc. Cat#: 09366-44 Fibroblast Growth Medium 2 Kit PromoCell Cat#: C23020 Dulbecco’s Phosphate Buffered Saline (DPBS) Nacalai Tesque Inc. Cat#: 14249-24 4%-Paraformaldehyde Phosphate Buffer Solution (PFA) Nacalai Tesque Inc. Cat#: 09154-85 (Continued on next page) Cell Reports 45, 116740, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-seq data This paper GEO accession #: GSE286568 Experimental models: Cell lines HeLa ATCC Cat#: CCL-2; RRID: CVCL_0030 HeLa BAK/BAX DKO This paper N/A HeLa CASP8 KO This paper N/A HeLa IFNGR1 KO This paper N/A HeLa IFNGR2 KO This paper N/A HeLa IFNGR1/2 DKO This paper N/A HeLa TNFR1 KO This paper N/A A549 ATCC Cat#: CCL-185; RRID: CVCL_0023 RAW 264.7 ATCC Cat#: TIB-71; RRID: CVCL_0493 L929 ATCC Cat#: CCL-1; RRID: CVCL_0462 B16-F10 ATCC Cat#: CRL-6475; RRID: CVCL_0159 Vero ATCC Cat#: CCL-81; RRID: CVCL_0059 BHK-21 ATCC Cat#: CCL-10; RRID: CVCL_1914 HEK293T ATCC Cat#: CRL-3216; RRID: CVCL_0063 Experimental models: Organisms/strains C57BL/6 mice CLEA Japan, Inc. Cat#: C57BL/6JJcl Oligonucleotides See Table S3 for a complete list of oligonucleotides.

    Variant Assay:

    Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH Santa Cruz Biotechnology Cat#: sc-32233; RRID: AB_627679 Anti-caspase-8 Cell Signaling Technology Cat#: 9746; RRID:AB_2275120 Anti-Cleaved PARP (Asp214) Cell Signaling Technology Cat#: 9541; RRID:AB_331426 Anti-3B (Anti-VPg EMCV ) This paper (Created by Immuno-Biological Laboratories Co., Ltd.) N/A (see Method Details) Anti-Mengo 3DPol Santa Cruz Biotechnology Cat#: sc-65633; RRID:AB_1565377 Anti-iNOS Santa Cruz Biotechnology Cat#: sc-7271; RRID:AB_627810 Anti-STAT1 Cell Signaling Technology Cat#: 9172; RRID:AB_2198300 Anti-pSTAT1 (Y701) Cell Signaling Technology Cat#: 9167; RRID:AB_561284 Anti-pSTAT2 (Y690) Cell Signaling Technology Cat#: 4441; RRID: AB_2198445 Anti-Myc-Tag Cell Signaling Technology Cat#: 2276; RRID: AB_331783 Anti-IκBα Santa Cruz Biotechnology Cat#: sc-371; RRID: AB_2235952 Anti-HSP70 Santa Cruz Biotechnology Cat#: sc-32239; RRID: AB_627759 Anti-IFNGR1 Proteintech Cat#: 10808-1-AP; RRID: AB_2121604 Anti-IFNGR2 Proteintech Cat#: 10266-1-AP; RRID: AB_2121709 Anti-mouse IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7056; RRID:AB_330921 Anti-rabbit IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7054; RRID: AB_2099235 Bacterial and virus strains Encephalomyocarditis virus (EMCV) ATCC Cat#: VR-129B Sendai Virus (SeV), strain Cantell ATCC Cat#: VR-907 Newcastle Disease Virus (NDV), strain Miyadera/51 Fujita et al., 1979 33 N/A Vesicular Stomatitis Virus (VSV), strain Indiana Oh et al., 2016 34 N/A Poliovirus (PV), strain type I Mahoney Shiroki et al., 1995 35 N/A Coxsackie Virus B3 (CVB3), strain Nancy Klump et al., 1990 36 N/A Enterovirus 71 (EV71), strain Isehara VP1145Q variant Kobayashi et al., 2018 37 N/A Competent high DH5α TOYOBO Cat#: DNA-903F Biological samples Human Dermal Fibroblasts, adult (HDFa) PromoCell Cat#: C-12302 Embryonated Chicken Eggs (9-day old) SHIMIZU Laboratory Supplies Co., Ltd. N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Nacalai Tesque Inc. Cat#: 16971-55 Fetal bovine serum (FBS) Sigma-Aldrich Cat#: 173012 Antibiotic-Antimycotic Mixed Stock Solution Nacalai Tesque Inc. Cat#: 09366-44 Fibroblast Growth Medium 2 Kit PromoCell Cat#: C23020 Dulbecco’s Phosphate Buffered Saline (DPBS) Nacalai Tesque Inc. Cat#: 14249-24 4%-Paraformaldehyde Phosphate Buffer Solution (PFA) Nacalai Tesque Inc. Cat#: 09154-85 (Continued on next page) Cell Reports 45, 116740, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-seq data This paper GEO accession #: GSE286568 Experimental models: Cell lines HeLa ATCC Cat#: CCL-2; RRID: CVCL_0030 HeLa BAK/BAX DKO This paper N/A HeLa CASP8 KO This paper N/A HeLa IFNGR1 KO This paper N/A HeLa IFNGR2 KO This paper N/A HeLa IFNGR1/2 DKO This paper N/A HeLa TNFR1 KO This paper N/A A549 ATCC Cat#: CCL-185; RRID: CVCL_0023 RAW 264.7 ATCC Cat#: TIB-71; RRID: CVCL_0493 L929 ATCC Cat#: CCL-1; RRID: CVCL_0462 B16-F10 ATCC Cat#: CRL-6475; RRID: CVCL_0159 Vero ATCC Cat#: CCL-81; RRID: CVCL_0059 BHK-21 ATCC Cat#: CCL-10; RRID: CVCL_1914 HEK293T ATCC Cat#: CRL-3216; RRID: CVCL_0063 Experimental models: Organisms/strains C57BL/6 mice CLEA Japan, Inc. Cat#: C57BL/6JJcl Oligonucleotides See Table S3 for a complete list of oligonucleotides.

    Recombinant:

    Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH Santa Cruz Biotechnology Cat#: sc-32233; RRID: AB_627679 Anti-caspase-8 Cell Signaling Technology Cat#: 9746; RRID:AB_2275120 Anti-Cleaved PARP (Asp214) Cell Signaling Technology Cat#: 9541; RRID:AB_331426 Anti-3B (Anti-VPg EMCV ) This paper (Created by Immuno-Biological Laboratories Co., Ltd.) N/A (see Method Details) Anti-Mengo 3DPol Santa Cruz Biotechnology Cat#: sc-65633; RRID:AB_1565377 Anti-iNOS Santa Cruz Biotechnology Cat#: sc-7271; RRID:AB_627810 Anti-STAT1 Cell Signaling Technology Cat#: 9172; RRID:AB_2198300 Anti-pSTAT1 (Y701) Cell Signaling Technology Cat#: 9167; RRID:AB_561284 Anti-pSTAT2 (Y690) Cell Signaling Technology Cat#: 4441; RRID: AB_2198445 Anti-Myc-Tag Cell Signaling Technology Cat#: 2276; RRID: AB_331783 Anti-IκBα Santa Cruz Biotechnology Cat#: sc-371; RRID: AB_2235952 Anti-HSP70 Santa Cruz Biotechnology Cat#: sc-32239; RRID: AB_627759 Anti-IFNGR1 Proteintech Cat#: 10808-1-AP; RRID: AB_2121604 Anti-IFNGR2 Proteintech Cat#: 10266-1-AP; RRID: AB_2121709 Anti-mouse IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7056; RRID:AB_330921 Anti-rabbit IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7054; RRID: AB_2099235 Bacterial and virus strains Encephalomyocarditis virus (EMCV) ATCC Cat#: VR-129B Sendai Virus (SeV), strain Cantell ATCC Cat#: VR-907 Newcastle Disease Virus (NDV), strain Miyadera/51 Fujita et al., 1979 33 N/A Vesicular Stomatitis Virus (VSV), strain Indiana Oh et al., 2016 34 N/A Poliovirus (PV), strain type I Mahoney Shiroki et al., 1995 35 N/A Coxsackie Virus B3 (CVB3), strain Nancy Klump et al., 1990 36 N/A Enterovirus 71 (EV71), strain Isehara VP1145Q variant Kobayashi et al., 2018 37 N/A Competent high DH5α TOYOBO Cat#: DNA-903F Biological samples Human Dermal Fibroblasts, adult (HDFa) PromoCell Cat#: C-12302 Embryonated Chicken Eggs (9-day old) SHIMIZU Laboratory Supplies Co., Ltd. N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Nacalai Tesque Inc. Cat#: 16971-55 Fetal bovine serum (FBS) Sigma-Aldrich Cat#: 173012 Antibiotic-Antimycotic Mixed Stock Solution Nacalai Tesque Inc. Cat#: 09366-44 Fibroblast Growth Medium 2 Kit PromoCell Cat#: C23020 Dulbecco’s Phosphate Buffered Saline (DPBS) Nacalai Tesque Inc. Cat#: 14249-24 4%-Paraformaldehyde Phosphate Buffer Solution (PFA) Nacalai Tesque Inc. Cat#: 09154-85 (Continued on next page) Cell Reports 45, 116740, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-seq data This paper GEO accession #: GSE286568 Experimental models: Cell lines HeLa ATCC Cat#: CCL-2; RRID: CVCL_0030 HeLa BAK/BAX DKO This paper N/A HeLa CASP8 KO This paper N/A HeLa IFNGR1 KO This paper N/A HeLa IFNGR2 KO This paper N/A HeLa IFNGR1/2 DKO This paper N/A HeLa TNFR1 KO This paper N/A A549 ATCC Cat#: CCL-185; RRID: CVCL_0023 RAW 264.7 ATCC Cat#: TIB-71; RRID: CVCL_0493 L929 ATCC Cat#: CCL-1; RRID: CVCL_0462 B16-F10 ATCC Cat#: CRL-6475; RRID: CVCL_0159 Vero ATCC Cat#: CCL-81; RRID: CVCL_0059 BHK-21 ATCC Cat#: CCL-10; RRID: CVCL_1914 HEK293T ATCC Cat#: CRL-3216; RRID: CVCL_0063 Experimental models: Organisms/strains C57BL/6 mice CLEA Japan, Inc. Cat#: C57BL/6JJcl Oligonucleotides See Table S3 for a complete list of oligonucleotides.

    Modification:

    Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH Santa Cruz Biotechnology Cat#: sc-32233; RRID: AB_627679 Anti-caspase-8 Cell Signaling Technology Cat#: 9746; RRID:AB_2275120 Anti-Cleaved PARP (Asp214) Cell Signaling Technology Cat#: 9541; RRID:AB_331426 Anti-3B (Anti-VPg EMCV ) This paper (Created by Immuno-Biological Laboratories Co., Ltd.) N/A (see Method Details) Anti-Mengo 3DPol Santa Cruz Biotechnology Cat#: sc-65633; RRID:AB_1565377 Anti-iNOS Santa Cruz Biotechnology Cat#: sc-7271; RRID:AB_627810 Anti-STAT1 Cell Signaling Technology Cat#: 9172; RRID:AB_2198300 Anti-pSTAT1 (Y701) Cell Signaling Technology Cat#: 9167; RRID:AB_561284 Anti-pSTAT2 (Y690) Cell Signaling Technology Cat#: 4441; RRID: AB_2198445 Anti-Myc-Tag Cell Signaling Technology Cat#: 2276; RRID: AB_331783 Anti-IκBα Santa Cruz Biotechnology Cat#: sc-371; RRID: AB_2235952 Anti-HSP70 Santa Cruz Biotechnology Cat#: sc-32239; RRID: AB_627759 Anti-IFNGR1 Proteintech Cat#: 10808-1-AP; RRID: AB_2121604 Anti-IFNGR2 Proteintech Cat#: 10266-1-AP; RRID: AB_2121709 Anti-mouse IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7056; RRID:AB_330921 Anti-rabbit IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7054; RRID: AB_2099235 Bacterial and virus strains Encephalomyocarditis virus (EMCV) ATCC Cat#: VR-129B Sendai Virus (SeV), strain Cantell ATCC Cat#: VR-907 Newcastle Disease Virus (NDV), strain Miyadera/51 Fujita et al., 1979 33 N/A Vesicular Stomatitis Virus (VSV), strain Indiana Oh et al., 2016 34 N/A Poliovirus (PV), strain type I Mahoney Shiroki et al., 1995 35 N/A Coxsackie Virus B3 (CVB3), strain Nancy Klump et al., 1990 36 N/A Enterovirus 71 (EV71), strain Isehara VP1145Q variant Kobayashi et al., 2018 37 N/A Competent high DH5α TOYOBO Cat#: DNA-903F Biological samples Human Dermal Fibroblasts, adult (HDFa) PromoCell Cat#: C-12302 Embryonated Chicken Eggs (9-day old) SHIMIZU Laboratory Supplies Co., Ltd. N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Nacalai Tesque Inc. Cat#: 16971-55 Fetal bovine serum (FBS) Sigma-Aldrich Cat#: 173012 Antibiotic-Antimycotic Mixed Stock Solution Nacalai Tesque Inc. Cat#: 09366-44 Fibroblast Growth Medium 2 Kit PromoCell Cat#: C23020 Dulbecco’s Phosphate Buffered Saline (DPBS) Nacalai Tesque Inc. Cat#: 14249-24 4%-Paraformaldehyde Phosphate Buffer Solution (PFA) Nacalai Tesque Inc. Cat#: 09154-85 (Continued on next page) Cell Reports 45, 116740, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-seq data This paper GEO accession #: GSE286568 Experimental models: Cell lines HeLa ATCC Cat#: CCL-2; RRID: CVCL_0030 HeLa BAK/BAX DKO This paper N/A HeLa CASP8 KO This paper N/A HeLa IFNGR1 KO This paper N/A HeLa IFNGR2 KO This paper N/A HeLa IFNGR1/2 DKO This paper N/A HeLa TNFR1 KO This paper N/A A549 ATCC Cat#: CCL-185; RRID: CVCL_0023 RAW 264.7 ATCC Cat#: TIB-71; RRID: CVCL_0493 L929 ATCC Cat#: CCL-1; RRID: CVCL_0462 B16-F10 ATCC Cat#: CRL-6475; RRID: CVCL_0159 Vero ATCC Cat#: CCL-81; RRID: CVCL_0059 BHK-21 ATCC Cat#: CCL-10; RRID: CVCL_1914 HEK293T ATCC Cat#: CRL-3216; RRID: CVCL_0063 Experimental models: Organisms/strains C57BL/6 mice CLEA Japan, Inc. Cat#: C57BL/6JJcl Oligonucleotides See Table S3 for a complete list of oligonucleotides.

    Saline:

    Article Title: A 20-amino-acid Cardiovirus protein exhibits cytokine-mimicry activity to regulate viral replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GAPDH Santa Cruz Biotechnology Cat#: sc-32233; RRID: AB_627679 Anti-caspase-8 Cell Signaling Technology Cat#: 9746; RRID:AB_2275120 Anti-Cleaved PARP (Asp214) Cell Signaling Technology Cat#: 9541; RRID:AB_331426 Anti-3B (Anti-VPg EMCV ) This paper (Created by Immuno-Biological Laboratories Co., Ltd.) N/A (see Method Details) Anti-Mengo 3DPol Santa Cruz Biotechnology Cat#: sc-65633; RRID:AB_1565377 Anti-iNOS Santa Cruz Biotechnology Cat#: sc-7271; RRID:AB_627810 Anti-STAT1 Cell Signaling Technology Cat#: 9172; RRID:AB_2198300 Anti-pSTAT1 (Y701) Cell Signaling Technology Cat#: 9167; RRID:AB_561284 Anti-pSTAT2 (Y690) Cell Signaling Technology Cat#: 4441; RRID: AB_2198445 Anti-Myc-Tag Cell Signaling Technology Cat#: 2276; RRID: AB_331783 Anti-IκBα Santa Cruz Biotechnology Cat#: sc-371; RRID: AB_2235952 Anti-HSP70 Santa Cruz Biotechnology Cat#: sc-32239; RRID: AB_627759 Anti-IFNGR1 Proteintech Cat#: 10808-1-AP; RRID: AB_2121604 Anti-IFNGR2 Proteintech Cat#: 10266-1-AP; RRID: AB_2121709 Anti-mouse IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7056; RRID:AB_330921 Anti-rabbit IgG, AP-linked (secondary antibody) Cell Signaling Technology Cat#: 7054; RRID: AB_2099235 Bacterial and virus strains Encephalomyocarditis virus (EMCV) ATCC Cat#: VR-129B Sendai Virus (SeV), strain Cantell ATCC Cat#: VR-907 Newcastle Disease Virus (NDV), strain Miyadera/51 Fujita et al., 1979 33 N/A Vesicular Stomatitis Virus (VSV), strain Indiana Oh et al., 2016 34 N/A Poliovirus (PV), strain type I Mahoney Shiroki et al., 1995 35 N/A Coxsackie Virus B3 (CVB3), strain Nancy Klump et al., 1990 36 N/A Enterovirus 71 (EV71), strain Isehara VP1145Q variant Kobayashi et al., 2018 37 N/A Competent high DH5α TOYOBO Cat#: DNA-903F Biological samples Human Dermal Fibroblasts, adult (HDFa) PromoCell Cat#: C-12302 Embryonated Chicken Eggs (9-day old) SHIMIZU Laboratory Supplies Co., Ltd. N/A Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle Medium (DMEM) Nacalai Tesque Inc. Cat#: 16971-55 Fetal bovine serum (FBS) Sigma-Aldrich Cat#: 173012 Antibiotic-Antimycotic Mixed Stock Solution Nacalai Tesque Inc. Cat#: 09366-44 Fibroblast Growth Medium 2 Kit PromoCell Cat#: C23020 Dulbecco’s Phosphate Buffered Saline (DPBS) Nacalai Tesque Inc. Cat#: 14249-24 4%-Paraformaldehyde Phosphate Buffer Solution (PFA) Nacalai Tesque Inc. Cat#: 09154-85 (Continued on next page) Cell Reports 45, 116740, January 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA-seq data This paper GEO accession #: GSE286568 Experimental models: Cell lines HeLa ATCC Cat#: CCL-2; RRID: CVCL_0030 HeLa BAK/BAX DKO This paper N/A HeLa CASP8 KO This paper N/A HeLa IFNGR1 KO This paper N/A HeLa IFNGR2 KO This paper N/A HeLa IFNGR1/2 DKO This paper N/A HeLa TNFR1 KO This paper N/A A549 ATCC Cat#: CCL-185; RRID: CVCL_0023 RAW 264.7 ATCC Cat#: TIB-71; RRID: CVCL_0493 L929 ATCC Cat#: CCL-1; RRID: CVCL_0462 B16-F10 ATCC Cat#: CRL-6475; RRID: CVCL_0159 Vero ATCC Cat#: CCL-81; RRID: CVCL_0059 BHK-21 ATCC Cat#: CCL-10; RRID: CVCL_1914 HEK293T ATCC Cat#: CRL-3216; RRID: CVCL_0063 Experimental models: Organisms/strains C57BL/6 mice CLEA Japan, Inc. Cat#: C57BL/6JJcl Oligonucleotides See Table S3 for a complete list of oligonucleotides.



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    ATCC virus strains sendai virus cantell strain atcc cat
    Figure 4. Phosphorylation of MAVS is important for type I IFN induction and for antiviral defense against infection by RNA viruses (A and B) HEK293T cells transiently transfected for 20 h with expression vectors for Myc-tagged WT mMAVS or Myc-tagged mMAVS S186A/S220A (2SA) were infected with <t>Sendai</t> virus (SeV) or Vesicular Stomatitis Virus (VSV), and then subjected to immunoprecipitation (IP) with an anti-Myc antibody. The resulting precipitates were then subjected to immunoblot analysis, together with the original cell lysates (Total), with antibodies specific for phospho-S 220 MAVS, Myc, p-p38, or p38 (A). Results are representative of four independent experiments. Densitometry analysis of the phospho-S 220 MAVS band relative to the Myc band in the immunoprecipitate (B). Data are expressed as the mean ± SEM from four independent experiments (*p < 0.05, two-tailed Student’s t test). (C–E) MAVS KO MEFs reconstituted with MAVS WT or the 2SA mutant were infected with SeV for 3 h. Next, levels of Ifna (C), Ifnb1 (D), or Sendai virus P (E) mRNA were measured by reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis. Data are expressed as the mean ± SEM of four in- dependent experiments (*p < 0.05, two-tailed Student’s t test).
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    Figure 4. Phosphorylation of MAVS is important for type I IFN induction and for antiviral defense against infection by RNA viruses (A and B) HEK293T cells transiently transfected for 20 h with expression vectors for Myc-tagged WT mMAVS or Myc-tagged mMAVS S186A/S220A (2SA) were infected with Sendai virus (SeV) or Vesicular Stomatitis Virus (VSV), and then subjected to immunoprecipitation (IP) with an anti-Myc antibody. The resulting precipitates were then subjected to immunoblot analysis, together with the original cell lysates (Total), with antibodies specific for phospho-S 220 MAVS, Myc, p-p38, or p38 (A). Results are representative of four independent experiments. Densitometry analysis of the phospho-S 220 MAVS band relative to the Myc band in the immunoprecipitate (B). Data are expressed as the mean ± SEM from four independent experiments (*p < 0.05, two-tailed Student’s t test). (C–E) MAVS KO MEFs reconstituted with MAVS WT or the 2SA mutant were infected with SeV for 3 h. Next, levels of Ifna (C), Ifnb1 (D), or Sendai virus P (E) mRNA were measured by reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis. Data are expressed as the mean ± SEM of four in- dependent experiments (*p < 0.05, two-tailed Student’s t test).

    Journal: iScience

    Article Title: MAVS phosphorylation acts as a cellular stress sensor that modulates antiviral immunity

    doi: 10.1016/j.isci.2025.113256

    Figure Lengend Snippet: Figure 4. Phosphorylation of MAVS is important for type I IFN induction and for antiviral defense against infection by RNA viruses (A and B) HEK293T cells transiently transfected for 20 h with expression vectors for Myc-tagged WT mMAVS or Myc-tagged mMAVS S186A/S220A (2SA) were infected with Sendai virus (SeV) or Vesicular Stomatitis Virus (VSV), and then subjected to immunoprecipitation (IP) with an anti-Myc antibody. The resulting precipitates were then subjected to immunoblot analysis, together with the original cell lysates (Total), with antibodies specific for phospho-S 220 MAVS, Myc, p-p38, or p38 (A). Results are representative of four independent experiments. Densitometry analysis of the phospho-S 220 MAVS band relative to the Myc band in the immunoprecipitate (B). Data are expressed as the mean ± SEM from four independent experiments (*p < 0.05, two-tailed Student’s t test). (C–E) MAVS KO MEFs reconstituted with MAVS WT or the 2SA mutant were infected with SeV for 3 h. Next, levels of Ifna (C), Ifnb1 (D), or Sendai virus P (E) mRNA were measured by reverse transcription and quantitative polymerase chain reaction (RT-qPCR) analysis. Data are expressed as the mean ± SEM of four in- dependent experiments (*p < 0.05, two-tailed Student’s t test).

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-phosphoS220 MAVS Antibody Eurofins acetyl-TYGPV(phospho)SPTVS anti-Myc (9E10) Santa Cruz Technology sc-40; RRID:AB_627268 anti-HA(Y-11) Santa Cruz Technology sc-805; RRID:AB_631618 anti-p38(c-20) Santa Cruz Technology sc-535; RRID:AB_632138 anti–phospho-p38 (Thr180/Tyr182) Cell Signaling 9211; RRID:AB_331641 anti–cleaved caspase-3 (D175) 5A1E Cell Signaling 9664; RRID:AB_2070042 anti-MAVS (rodent specific) Cell Signaling 4983; RRID:AB_823566 anti–phospho-IRF3 (Ser396) 4D4G Cell Signaling 4947; RRID:AB_823547 anti-TBK1/NAK (D1B4) Cell Signaling 3504; RRID:AB_2255663 rabbit polyclonal anti-Flag Rockland Inc. 600-401-383; RRID:AB_219374 Amersham, ECL Rabbit IgG, HRP-linked whole Ab (from donkey) Cytiva NA934; RRID:AB_772206 Amersham, ECL Mouse IgG, HRP-linked whole Ab (from sheep) Cytiva NA931; RRID:AB_772210 Bacterial and virus strains Sendai virus American Type Culture Collection Cantell strain, VR-907 Vesicular stomatitis virus-green fluorescent protein (VSV-GFP) This paper Chemicals, peptides, and recombinant proteins SB202190 Med Chem Express HY-10295 SP600125 Sigma Aldrich S5567-10 MG Rotenone Sigma Aldrich R8875-1G Thapsigargin Tocris 1138 Tunicamycin LKT Labs, Inc. T8153 RNAiso TaKaRa 9108 Dulbecco’s Modified Eagle’s Medium (DMEM) Sigma Aldrich D6429 Fetal bovine serum Gibco 10437028 Penicillin-streptomycin Nacalai Tesque Inc 06168–34 Lipofectamine Transfection Reagent Invitrogen 18324020 Poly(I:C) GE Healthcare 11545784 Lipofectamine 2000 Invitrogen 11668019 GeneJuice Merck Millipore 70967 Phos-stop Roche 4906845001 Calf Intestinal Alkaline Phosphatase Takara Puromycin Sigma Aldrich P-8833 Critical commercial assays ReverTra Ace TM qPCR RT Master Mix Toyobo FSQ301 KAPA SYBR® FAST qPCR Master Mix NIPPON Genetics Co. Ltd KK4602 Dual-Luciferase® Reporter Assay System Promega E1910 Deposited data Proteomic identification of phosphorylation sites of MAVS This study https://repository.jpostdb.org/ entry/JPST003870.0 PXD064922 (Continued on next page) iScience 28, 113256, September 19, 2025 e1

    Techniques: Phospho-proteomics, Infection, Transfection, Expressing, Virus, Immunoprecipitation, Western Blot, Two Tailed Test, Mutagenesis, Reverse Transcription, Real-time Polymerase Chain Reaction, Quantitative RT-PCR